Wound healing is a well-coordinated sequence of events in which signaling and remodeling of the extracellular matrix (ECM) are integral components. dehydrated human amnion/chorion membrane (dHACM) has demonstrated clinical efficacy in the enhancement of wound repair compared with standard compression therapy. Jennifer Ley and Coll conducted this study to characterize components that function in wound healing, including inhibitor and protease content and activity, in micronized dHACM. This study identified ECM components, growth factors, cytokines, and MMPs/inhibitors in micronized dHACM that can play a role in the wound healing process.
The treatment of large, chronic wounds (e.g., foot ulcers, venous leg ulcers) is challenging, particularly in diabetic patient populations. Physiological wound healing is a well-coordinated sequence of events in which signalling and remodelling of the extracellular matrix (ECM) are integral components. After an injury, the wound progresses through hemostasis, inflammation, proliferation, and repair/remodelling, ultimately resulting in the regeneration of functional tissue.
The amniotic membrane has a long history of use in wound-healing applications due to the dynamic nature of the tissue and its growth factor/cytokine rich ECM. In particular, dehydrated human amnion/chorion membrane (dHACM) has demonstrated clinical efficacy in the enhancement of wound repair compared with standard compression therapy. dHACM tissue contained the ECM components collagens I and IV, hyaluronic acid, heparin sulfate proteoglycans, fibronectin, and laminin, numerous growth factors, cytokines, chemokines, proteases, and protease inhibitors that are known to play a role in the wound-healing process were quantified in dHACM
The treatment of complex, full-thickness wounds may be augmented by the use of a micronized formulation of dHACM (in addition to membrane dHACM) to fill the wound bed and to provide additional bioactive molecules to the site of injury. Membrane and micronized formulations of dehydrated human amnion/chorion membranes (dHACM, EpiFix; MiMedx Group, Inc.) processed by a proprietary PURION Process19were used in this study
Jennifer Ley and Coll conducted this study to characterize components that function in wound healing, including inhibitor and protease content and activity, in micronized dHACM. To visualize ECM components found in dHACM tissue, immunofluorescent staining was performed for collagen IV, collagen I, and HA. This study identified ECM components, growth factors, cytokines, and MMPs/inhibitors in micronized dHACM that can play a role in the wound healing process
Due to the dynamic nature of the tissue and its biologic factor-rich ECM, amniotic membranes have demonstrated efficacy in wound-healing applications. These results identified ECM components and characterized a variety of growth factors, cytokines, proteases, and inhibitors present in the dHACM tissue.
Ultimately, the use of micronized dHACM in the treatment of chronic wounds may advance the current standards of wound care.
Authors: Jennifer Lei,*,{ Lauren B. Priddy,{ Jeremy J. Lim, Michelle Massee, and Thomas J. Koob
Newspaper: Adv Wound Care (New Rochelle). 2017 Feb 1; 6(2): 43–53.
Source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5286550/